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anti human muc16 alexa fluor 594  (R&D Systems)


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    Structured Review

    R&D Systems anti human muc16 alexa fluor 594
    Anti Human Muc16 Alexa Fluor 594, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ca125/Human+CA125%2FMUC16+Alexa+Fluor%C2%AE+594-conjugated+Antibody/pmc12972391-72-50-54
    Average 94 stars, based on 1 article reviews
    anti human muc16 alexa fluor 594 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Establishment of Novel High-Grade Serous Ovarian Carcinoma Cell Line OVAR79.
    Article Snippet: Cells were detached using 0.05% trypsin, washed three times with PBS, and fixed with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA). .. The cells were then placed in a serum-free protein block solution ((Dako/Agilent Technologies, San Diego, CA, USA) and incubated with primary antibodies—anti-CD324 (E-cadherin; Sony, Tokyo, Japan, #1336505), anti-EpCAM (Abcam, Cambridge, UK, ab223582), anti-CD44 (BD Biosciences, Franklin Lakes, NJ, USA, #2294010), anti-PanCK (Eagle BioSciences, Amherst, NH, USA, 10- 310015-01), anti-CA125 (R&D Systems, Minneapolis, MN, USA, mab56092), anti-Vimentin (Thermo Fisher Scientific, Waltham, MA, USA, RM-9120-S0), or anti-CD325 (N-cadherin; Sony, Tokyo, Japan, #2354010)—for 1 h, followed by a 1 h incubation with Alexa Fluor 488- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-11008, #A-11001), Alexa Fluor 555- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21434), or Alexa Fluor 647-conjugated secondary antibodies (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21235). .. After staining, samples were analyzed using a NovoCyte Flow Cytometer (ACEA Biosciences/Agilent Technologies, San Diego, CA, USA), and data were processed with NovoExpress Software (1.6.0).

    Article Title: Establishment of Novel High-Grade Serous Ovarian Carcinoma Cell Line OVAR79
    Article Snippet: Cells were detached using 0.05% trypsin, washed three times with PBS, and fixed with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA). .. The cells were then placed in a serum-free protein block solution ((Dako/Agilent Technologies, San Diego, CA, USA) and incubated with primary antibodies—anti-CD324 (E-cadherin; Sony, Tokyo, Japan, #1336505), anti-EpCAM (Abcam, Cambridge, UK, ab223582), anti-CD44 (BD Biosciences, Franklin Lakes, NJ, USA, #2294010), anti-PanCK (Eagle BioSciences, Amherst, NH, USA, 10-310015-01), anti-CA125 (R&D Systems, Minneapolis, MN, USA, mab56092), anti-Vimentin (Thermo Fisher Scientific, Waltham, MA, USA, RM-9120-S0), or anti-CD325 (N-cadherin; Sony, Tokyo, Japan, #2354010)—for 1 h, followed by a 1 h incubation with Alexa Fluor 488- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-11008, #A-11001), Alexa Fluor 555- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21434), or Alexa Fluor 647-conjugated secondary antibodies (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21235). .. After staining, samples were analyzed using a NovoCyte Flow Cytometer (ACEA Biosciences/Agilent Technologies, San Diego, CA, USA), and data were processed with NovoExpress Software (1.6.0).

    Incubation:

    Article Title: Establishment of Novel High-Grade Serous Ovarian Carcinoma Cell Line OVAR79.
    Article Snippet: Cells were detached using 0.05% trypsin, washed three times with PBS, and fixed with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA). .. The cells were then placed in a serum-free protein block solution ((Dako/Agilent Technologies, San Diego, CA, USA) and incubated with primary antibodies—anti-CD324 (E-cadherin; Sony, Tokyo, Japan, #1336505), anti-EpCAM (Abcam, Cambridge, UK, ab223582), anti-CD44 (BD Biosciences, Franklin Lakes, NJ, USA, #2294010), anti-PanCK (Eagle BioSciences, Amherst, NH, USA, 10- 310015-01), anti-CA125 (R&D Systems, Minneapolis, MN, USA, mab56092), anti-Vimentin (Thermo Fisher Scientific, Waltham, MA, USA, RM-9120-S0), or anti-CD325 (N-cadherin; Sony, Tokyo, Japan, #2354010)—for 1 h, followed by a 1 h incubation with Alexa Fluor 488- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-11008, #A-11001), Alexa Fluor 555- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21434), or Alexa Fluor 647-conjugated secondary antibodies (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21235). .. After staining, samples were analyzed using a NovoCyte Flow Cytometer (ACEA Biosciences/Agilent Technologies, San Diego, CA, USA), and data were processed with NovoExpress Software (1.6.0).

    Article Title: Improving SERS biosensors for the analysis of ovarian cancer-derived small extracellular vesicles.
    Article Snippet: Small extracellular vesicles (sEVs) are lipid bilayer vesicles that carry key molecules (e.g., proteins, DNAs, RNAs, and lipids) for cell-to-cell communication, being regarded as promising biomarkers for cancer diagnosis.. However, the detection of sEVs is still challenging due to their unique characteristics such as size and phenotype heterogeneity.. The surface-enhanced Raman scattering (SERS) assay is a promising tool for sEV analysis as it shows the advantages of robustness, high sensitivity, and specificity.

    Article Title: Establishment of Novel High-Grade Serous Ovarian Carcinoma Cell Line OVAR79
    Article Snippet: Cells were detached using 0.05% trypsin, washed three times with PBS, and fixed with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA). .. The cells were then placed in a serum-free protein block solution ((Dako/Agilent Technologies, San Diego, CA, USA) and incubated with primary antibodies—anti-CD324 (E-cadherin; Sony, Tokyo, Japan, #1336505), anti-EpCAM (Abcam, Cambridge, UK, ab223582), anti-CD44 (BD Biosciences, Franklin Lakes, NJ, USA, #2294010), anti-PanCK (Eagle BioSciences, Amherst, NH, USA, 10-310015-01), anti-CA125 (R&D Systems, Minneapolis, MN, USA, mab56092), anti-Vimentin (Thermo Fisher Scientific, Waltham, MA, USA, RM-9120-S0), or anti-CD325 (N-cadherin; Sony, Tokyo, Japan, #2354010)—for 1 h, followed by a 1 h incubation with Alexa Fluor 488- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-11008, #A-11001), Alexa Fluor 555- (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21434), or Alexa Fluor 647-conjugated secondary antibodies (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA, #A-21235). .. After staining, samples were analyzed using a NovoCyte Flow Cytometer (ACEA Biosciences/Agilent Technologies, San Diego, CA, USA), and data were processed with NovoExpress Software (1.6.0).

    Concentration Assay:

    Article Title: Improving SERS biosensors for the analysis of ovarian cancer-derived small extracellular vesicles.
    Article Snippet: Small extracellular vesicles (sEVs) are lipid bilayer vesicles that carry key molecules (e.g., proteins, DNAs, RNAs, and lipids) for cell-to-cell communication, being regarded as promising biomarkers for cancer diagnosis.. However, the detection of sEVs is still challenging due to their unique characteristics such as size and phenotype heterogeneity.. The surface-enhanced Raman scattering (SERS) assay is a promising tool for sEV analysis as it shows the advantages of robustness, high sensitivity, and specificity.

    Control:

    Article Title: Improving SERS biosensors for the analysis of ovarian cancer-derived small extracellular vesicles.
    Article Snippet: Small extracellular vesicles (sEVs) are lipid bilayer vesicles that carry key molecules (e.g., proteins, DNAs, RNAs, and lipids) for cell-to-cell communication, being regarded as promising biomarkers for cancer diagnosis.. However, the detection of sEVs is still challenging due to their unique characteristics such as size and phenotype heterogeneity.. The surface-enhanced Raman scattering (SERS) assay is a promising tool for sEV analysis as it shows the advantages of robustness, high sensitivity, and specificity.

    In Vitro:

    Article Title: Improving SERS biosensors for the analysis of ovarian cancer-derived small extracellular vesicles.
    Article Snippet: Small extracellular vesicles (sEVs) are lipid bilayer vesicles that carry key molecules (e.g., proteins, DNAs, RNAs, and lipids) for cell-to-cell communication, being regarded as promising biomarkers for cancer diagnosis.. However, the detection of sEVs is still challenging due to their unique characteristics such as size and phenotype heterogeneity.. The surface-enhanced Raman scattering (SERS) assay is a promising tool for sEV analysis as it shows the advantages of robustness, high sensitivity, and specificity.

    Suspension:

    Article Title: Improving SERS biosensors for the analysis of ovarian cancer-derived small extracellular vesicles.
    Article Snippet: Small extracellular vesicles (sEVs) are lipid bilayer vesicles that carry key molecules (e.g., proteins, DNAs, RNAs, and lipids) for cell-to-cell communication, being regarded as promising biomarkers for cancer diagnosis.. However, the detection of sEVs is still challenging due to their unique characteristics such as size and phenotype heterogeneity.. The surface-enhanced Raman scattering (SERS) assay is a promising tool for sEV analysis as it shows the advantages of robustness, high sensitivity, and specificity.



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